MiR-182-5p regulates BCL2L12 and BCL2 expression in acute myeloid leukemia as a potential therapeutic target

Biomed Pharmacother. 2018 Jan;97:1189-1194. doi: 10.1016/j.biopha.2017.11.002. Epub 2017 Nov 11.

Abstract

The importance of microRNAs (miRNAs) are shown during various cancers including acute myeloid leukemia (AML). MiR-182-5p functions as an oncogene or a potential suppressive miRNA in cancers, but its expression and function in AML is unknown. The purpose is to investigate the roles of miR-182-5p in AML in this study. MiR-182-5p was examined in the blood samples of AML and it was found that miR-182-5p expression levels were higher in AML tissues than it in their normal controls, so did in the AML cells. BCL2L12 and BCL2 were predicted as target genes of miR-182-5p and verified using luciferase reporter assay. BCL2L12 and BCL2 mRNA and protein levels were up-regulated in the AML cells with miR-182-5p inhibition. Cellular function of miR-182-5p indicated that miR-182-5p suppression in AML cells could decrease cell proliferation and reverse cisplatin (DDP) resistance via targeting BCL2L12 and BCL2 expression. Inhibition of miR-182-5p promoted AML cell apoptosis by targeting BCL2 or BCL2L12. The study demonstrates that high levels of miR-182-5p in AML promotes cell proliferation and suppresses cell apoptosis by targeting BCL2L12 and BCL2.

Keywords: AML; Apoptosis; BCL2; BCL2L12; MiR-182-5p.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Apoptosis / genetics
  • Case-Control Studies
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Cisplatin / pharmacology
  • Down-Regulation
  • Drug Resistance, Neoplasm
  • Female
  • Gene Expression Regulation, Leukemic
  • Humans
  • Leukemia, Myeloid, Acute / drug therapy
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Muscle Proteins / genetics*
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • RNA, Messenger / metabolism
  • Up-Regulation

Substances

  • Antineoplastic Agents
  • BCL2 protein, human
  • BCL2L12 protein, human
  • MicroRNAs
  • Mirn182 microRNA, human
  • Muscle Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Cisplatin