Combination Strategy of Quantitative Proteomics Uncovers the Related Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model

Methods Mol Biol. 2018;1788:185-192. doi: 10.1007/7651_2017_88.

Abstract

Quantitative proteome analysis using iTRAQ is an important technique to find disease-related proteins. As an important component of tumor microenvironment, tissue interstitial fluid (TIF) has drawn a great attention for its potential as a source for exploration of the solid tumor biomarkers. On the basis of a mouse model of colorectal cancer (CRC) that was induced by the carcinogenetic reagents, we adopted a quantitative proteome analysis with iTRAQ to discover the CRC-related proteins in the TIFs and with MRM to evaluate the corresponding abundance changes in the individual mouse TIF and serum samples.

Keywords: AOM-DSS mouse model; Colorectal cancer; MRM; Serum biomarker; Tissue interstitial fluid; iTRAQ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azoxymethane
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / blood
  • Blood Proteins / analysis*
  • Chromatography, Liquid / methods
  • Colon / pathology*
  • Colorectal Neoplasms / blood
  • Colorectal Neoplasms / chemically induced
  • Colorectal Neoplasms / chemistry
  • Colorectal Neoplasms / pathology*
  • Dextran Sulfate
  • Disease Models, Animal
  • Extracellular Fluid / chemistry*
  • Humans
  • Male
  • Mass Spectrometry / methods
  • Mice
  • Mice, Inbred C57BL
  • Proteome / analysis*
  • Proteomics / methods*
  • Tumor Microenvironment*

Substances

  • Biomarkers, Tumor
  • Blood Proteins
  • Proteome
  • Dextran Sulfate
  • Azoxymethane