Interspecies homology of liver microsomal cytochrome P-450. A form of dog cytochrome P-450 (P-450-D1) crossreactive with antibodies to rat P-450-male

Biochem Pharmacol. 1989 Jan 15;38(2):235-40. doi: 10.1016/0006-2952(89)90032-4.


P-450-male is a male specific form of cytochrome P-450 in rat liver microsomes. Cytochrome P-450 crossreactive with anti-P-450-male antibodies was purified to an electrophoretical homogeneity from liver microsomes of male beagle dogs. The specific content of the purified cytochrome P-450 (P-450-D1) was 16.9 nmol/mg protein. The apparent monomeric molecular weight of P-450-D1 was 48,000, which was smaller than P-450-male (51,000). P-450-D1 showed similarities in spectral properties, N-terminal amino acid sequence, and catalytic activities with some limited exceptions: P-450-D1 did not catalyze 2 alpha-hydroxylation of testosterone and progesterone and catalyzed 21-hydroxylation of progesterone. Based on these results, we propose that P-450-D1 is a form of cytochrome P-450 in the same gene subfamily as P-450-male.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aryl Hydrocarbon Hydroxylases*
  • Cattle
  • Chromatography, High Pressure Liquid
  • Cytochrome P-450 Enzyme System / immunology*
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Male
  • Microsomes, Liver / enzymology*
  • Mixed Function Oxygenases / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Progesterone / metabolism
  • Rats
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Spectrum Analysis
  • Steroid Hydroxylases / metabolism


  • Progesterone
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Steroid Hydroxylases
  • Aryl Hydrocarbon Hydroxylases
  • testosterone 7-alpha-hydroxylase, hamster