Major nucleolar proteins shuttle between nucleus and cytoplasm

Cell. 1989 Feb 10;56(3):379-90. doi: 10.1016/0092-8674(89)90241-9.


Nucleolin is a 92 kd nucleolar protein implicated in regulating polymerase I transcription and binding of preribosomal RNA. Another abundant nucleolar protein of 38 kd (B23/No38) is thought to be involved in intranuclear packaging of preribosomal particles. Although both proteins have previously been detected only in nuclei, we conclude that they shuttle constantly between nucleus and cytoplasm. This conclusion is based on monitoring the equilibration of these proteins between nuclei present in interspecies heterokaryons, and on observing the antigen-mediated nuclear accumulation of cytoplasmically injected antibodies. Our unexpected results suggest a role for these major nucleolar proteins in the nucleocytoplasmic transport of ribosomal components. Moreover, they suggest that transient exposure of shuttling proteins to the cytoplasm may provide a mechanism for cytoplasmic regulation of nuclear activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Nucleolus / metabolism*
  • Cell Nucleus / metabolism*
  • Chick Embryo
  • Chickens
  • Cloning, Molecular
  • Cytoplasm / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Nucleophosmin
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • RNA-Binding Proteins*
  • Species Specificity
  • Xenopus


  • Npm1 protein, mouse
  • Nuclear Proteins
  • Phosphoproteins
  • RNA-Binding Proteins
  • nucleolin
  • Nucleophosmin

Associated data

  • GENBANK/M24093
  • GENBANK/M24094