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, 242 (2), 285-92

The Refined 2.0 A X-ray Crystal Structure of the Complex Formed Between Bovine Beta-Trypsin and CMTI-I, a Trypsin Inhibitor From Squash Seeds (Cucurbita Maxima). Topological Similarity of the Squash Seed Inhibitors With the Carboxypeptidase A Inhibitor From Potatoes

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The Refined 2.0 A X-ray Crystal Structure of the Complex Formed Between Bovine Beta-Trypsin and CMTI-I, a Trypsin Inhibitor From Squash Seeds (Cucurbita Maxima). Topological Similarity of the Squash Seed Inhibitors With the Carboxypeptidase A Inhibitor From Potatoes

W Bode et al. FEBS Lett.

Abstract

The stoichiometric complex formed between bovine beta-trypsin and the Cucurbita maxima trypsin inhibitor I (CMTI-I) was crystallized and its X-ray crystal structure determined using Patterson search techniques. Its structure has been crystallographically refined to a final R value of 0.152 (6.0-2.0 A). CMTI-I is of ellipsoidal shape; it lacks helices or beta-sheets, but consists of turns and connecting short polypeptide stretches. The disulfide pairing is CYS-3I-20I, Cys-10I-22I and Cys-16I-28I. According to the polypeptide fold and disulfide connectivity its structure resembles that of the carboxypeptidase A inhibitor from potatoes. Thirteen of the 29 inhibitor residues are in direct contact with trypsin; most of them are in the primary binding segment Val-2I (P4)-Glu-9I (P4') which contains the reactive site bond Arg-5I-Ile-6I and is in a conformation observed also for other serine proteinase inhibitors.

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