Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration

FASEB J. 1989 Feb;3(2):174-7. doi: 10.1096/fasebj.3.2.2914628.


Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / metabolism
  • Animals
  • Cells, Cultured
  • Collagen / physiology
  • Epithelium / physiology
  • Epithelium / ultrastructure
  • Extracellular Matrix / physiology*
  • Extracellular Matrix / ultrastructure
  • Liver / physiology*
  • Liver / ultrastructure
  • Rats


  • Albumins
  • Collagen