Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes

Laurent Poirel; Yu Larpin; Jan Dobias; Roger Stephan; Jean-Winoc Decousser; Jean-Yves Madec; Patrice Nordmann

doi:10.1016/j.diagmicrobio.2017.09.012

Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes

Diagn Microbiol Infect Dis. 2018 Jan;90(1):7-10. doi: 10.1016/j.diagmicrobio.2017.09.012. Epub 2017 Sep 22.

Authors

Laurent Poirel¹, Yu Larpin², Jan Dobias³, Roger Stephan⁴, Jean-Winoc Decousser⁵, Jean-Yves Madec⁶, Patrice Nordmann⁷

Affiliations

¹ Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland.
² Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland.
³ Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
⁴ Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland.
⁵ Department of Virology, Bacteriology - Infection Control, Parasitology - Mycology, Assistance Publique - Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France; IAME, UMR 1137, INSERM, Paris, France.
⁶ Agence Nationale de Sécurité Sanitaire (Anses), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
⁷ Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland; Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland. Electronic address: patrice.nordmann@unifr.ch.

PMID: 29146285
DOI: 10.1016/j.diagmicrobio.2017.09.012

Abstract

The Rapid Polymyxin NP test has been recently developed to rapidly detect polymyxin resistance in Enterobacteriaceae. Here we evaluated this test for detecting MCR-1/MCR-2-producing Enterobacteriaceae using a collection of 70 non-redundant strains either recovered from the environment, animals, or humans. Sensitivity and specificity were found to be 100%.

Keywords: Enterobacteriaceae; MCR; Polymyxin; Rapid Polymyxin NP test; Resistance.

Publication types

Evaluation Study

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Colistin / pharmacology*
Drug Resistance, Bacterial / genetics*
Enterobacter aerogenes / drug effects
Enterobacter aerogenes / genetics
Enterobacter aerogenes / isolation & purification
Enterobacter cloacae / drug effects
Enterobacter cloacae / genetics
Enterobacter cloacae / isolation & purification
Enterobacteriaceae / drug effects*
Enterobacteriaceae / genetics*
Enterobacteriaceae Infections / drug therapy
Enterobacteriaceae Infections / microbiology
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / isolation & purification
Escherichia coli Proteins / genetics*
Humans
Klebsiella oxytoca / drug effects
Klebsiella oxytoca / genetics
Klebsiella oxytoca / isolation & purification
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Membrane Proteins / genetics*
Microbial Sensitivity Tests
Polymyxins / pharmacology*
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-1 protein, E coli
Membrane Proteins
Polymyxins
mcr-2 protein, E coli
beta-Lactamases
Colistin