High-Quality Draft Genome Sequence of " Candidatus Methanoperedens sp." Strain BLZ2, a Nitrate-Reducing Anaerobic Methane-Oxidizing Archaeon Enriched in an Anoxic Bioreactor

Stefanie Berger; Jeroen Frank; Paula Dalcin Martins; Mike S M Jetten; Cornelia U Welte

doi:10.1128/genomeA.01159-17

High-Quality Draft Genome Sequence of " Candidatus Methanoperedens sp." Strain BLZ2, a Nitrate-Reducing Anaerobic Methane-Oxidizing Archaeon Enriched in an Anoxic Bioreactor

Genome Announc. 2017 Nov 16;5(46):e01159-17. doi: 10.1128/genomeA.01159-17.

Authors

Stefanie Berger¹, Jeroen Frank¹, Paula Dalcin Martins², Mike S M Jetten¹, Cornelia U Welte³

Affiliations

¹ Department of Microbiology, Institute for Water and Wetland Research, Radboud University, Nijmegen, The Netherlands.
² Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
³ Department of Microbiology, Institute for Water and Wetland Research, Radboud University, Nijmegen, The Netherlands c.welte@science.ru.nl.

Abstract

The high-quality draft genome of "Candidatus Methanoperedens sp." strain BLZ2, a nitrate-reducing archaeon anaerobically oxidizing methane, is presented. The genome was obtained from an enrichment culture and measures 3.74 Mb. It harbors two nitrate reductase gene clusters, an ammonium-forming nitrite reductase, and the complete reverse methanogenesis pathway. Methane that escapes to the atmosphere acts as a potent greenhouse gas. Global methane emissions are mitigated by methanotrophs, which oxidize methane to CO₂ "Candidatus Methanoperedens spp." are unique methanotrophic archaea that can perform nitrate-dependent anaerobic oxidation of methane. A high-quality draft genome sequence of only 85 contigs from this archaeon is presented here.