Isolation, characterization, and synthesis of chrysobactin, a compound with siderophore activity from Erwinia chrysanthemi

J Biol Chem. 1989 Feb 25;264(6):3187-93.

Abstract

A catechcol-type siderophore, assigned the trivial name chrysobactin, was isolated from the phytopathogenic bacterium Erwinia chrysanthemi and characterized by degradation and spectroscopic techniques as N-[N2-(2,3-dihydroxybenzoyl)-D-lysyl]-L-serine. Chrysobactin, which was also obtained by chemical synthesis, was shown to be active in supplying iron to a group of mutants of E. chrysanthemi defective in biosynthesis of the siderophore.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Chemical Phenomena
  • Chemistry
  • Chromatography, High Pressure Liquid
  • Erwinia / analysis*
  • Erwinia / genetics
  • Erwinia / growth & development
  • Ethylenediamines
  • Hydrogen-Ion Concentration
  • Iron / metabolism
  • Magnetic Resonance Spectroscopy
  • Molecular Weight
  • Mutation
  • Spectrophotometry

Substances

  • Amino Acids
  • Ethylenediamines
  • ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid)
  • Iron