Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM

Structure. 2017 Dec 5;25(12):1856-1866.e2. doi: 10.1016/j.str.2017.10.008. Epub 2017 Nov 16.

Abstract

Talin mediates attachment of the cell to the extracellular matrix. It is targeted by the Rap1 effector RIAM to focal adhesion sites and subsequently undergoes force-induced conformational opening to recruit the actin-interacting protein vinculin. The conformational switch involves the talin R3 domain, which binds RIAM when closed and vinculin when open. Here, we apply pressure to R3 and measure 1H, 15N, and 13C chemical shift changes, which are fitted using a simple model, and indicate that R3 is only 50% closed: the closed form is a four-helix bundle, while in the open state helix 1 is twisted out. Strikingly, a mutant of R3 that binds RIAM with an affinity similar to wild-type but more weakly to vinculin is shown to be 0.84 kJ mol-1 more stable when closed. These results demonstrate that R3 is thermodynamically poised to bind either RIAM or vinculin, and thus constitutes a good mechanosensitive switch.

Keywords: cell adhesion; chemical shift; focal adhesion complex; hydrostatic pressure; singular value decomposition; talin; vinculin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Animals
  • Binding Sites
  • Hydrostatic Pressure*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Docking Simulation*
  • Molecular Dynamics Simulation
  • Protein Binding
  • Talin / chemistry*
  • Talin / metabolism
  • Vinculin / chemistry*
  • Vinculin / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Membrane Proteins
  • RIAM protein, mouse
  • Talin
  • Vinculin