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Comparative Study
. 2018 Feb 5;149:419-424.
doi: 10.1016/j.jpba.2017.11.036. Epub 2017 Nov 14.

Investigation of the Effect of Blood Hematocrit and Lipid Content on the Blood Volume Deposited by a Disposable Dried Blood Spot Collection Device

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Comparative Study

Investigation of the Effect of Blood Hematocrit and Lipid Content on the Blood Volume Deposited by a Disposable Dried Blood Spot Collection Device

N Spooner et al. J Pharm Biomed Anal. .

Abstract

When using dried blood spot (DBS) sampling for the quantitative bioanalysis of circulating concentrations of drugs, metabolites and endogenous analytes, it is important that a fixed volume of blood is deposited to overcome the issues associated with blood hematocrit (HCT) and homogeneity. The volumetric performance of the KTH DBS collection device was tested with radiolabelled [14C]-diclofenac. It was demonstrated that the device deposits a fixed volume of blood (13.5μL) regardless of the HCT (25-65%), or lipid content of the blood sample. Further, it was found that the precision and accuracy of the derived dried blood samples were at least as good as those of a positive displacement pipette. The device was found to be easy to use and gave acceptable dried samples for 92.9% of the tests performed (n=42).

Keywords: Bioanalysis; Dried blood spot; Hematocrit; Microsampling; Patient centric sampling.

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