MicroRNA let-7f-5p regulates neuronal differentiation of rat bone marrow mesenchymal stem cells by targeting Par6α

Biochem Biophys Res Commun. 2018 Jan 1;495(1):1476-1481. doi: 10.1016/j.bbrc.2017.11.024. Epub 2017 Nov 16.


Par6α (partitioning defective 6 homologue alpha), a component of the Par3/Par6/aPKC complex, was recently shown to be essential for axon specification during neuronal development. However, the biological functions and regulatory mechanisms of Par6α in the mesenchymal stem cell (MSC) differentiation process have not been investigated. In this study, we found that the expression of let-7f-5p was downregulated during differentiation of bone marrow-derived MSCs to neuron-like cells. Interestingly, Par6α was predicted to be a target gene of let-7f-5p by computerized analysis and the luciferase reporter assay. Using gain- and loss-of-function approaches, we found that expression of Par6α was inversely correlated with let-7f-5p levels during differentiation (p < 0.05). By silencing Par6α using siRNAs, we demonstrated that Par6α was necessary for MSC neuronal differentiation. Altogether, our studies proved that inhibition of let-7f-5p facilitates induction of MSCs into neuron-like cells by directly targeting Par6α.

Keywords: Axon differentiation; MSCs; Neuronal differentiation; Neuronal polarity; Par6.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Carrier Proteins / metabolism*
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Gene Expression Regulation, Developmental / physiology
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / physiology*
  • MicroRNAs / metabolism*
  • Neurogenesis / physiology
  • Neurons / cytology*
  • Neurons / physiology*
  • Rats
  • Rats, Sprague-Dawley


  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • MIRNLET7 microRNA, rat
  • MicroRNAs
  • Pard6a protein, rat