Altered Aconitase 2 Activity in Huntington's Disease Peripheral Blood Cells and Mouse Model Striatum

Int J Mol Sci. 2017 Nov 21;18(11):2480. doi: 10.3390/ijms18112480.


Huntington's disease (HD) is caused by an unstable cytosine adenine guanine (CAG) trinucleotide repeat expansion encoding a polyglutamine tract in the huntingtin protein. Previously, we identified several up- and down-regulated protein molecules in the striatum of the Hdh(CAG)150 knock-in mice at 16 months of age, a mouse model which is modeling the early human HD stage. Among those molecules, aconitase 2 (Aco2) located in the mitochondrial matrix is involved in the energy generation and susceptible to increased oxidative stress that would lead to inactivation of Aco2 activity. In this study, we demonstrate decreased Aco2 protein level and activity in the brain of both Hdh(CAG)150 and R6/2 mice. Aco2 activity was decreased in striatum of Hdh(CAG)150 mice at 16 months of age as well as R6/2 mice at 7 to 13 weeks of age. Aco2 activity in the striatum of R6/2 mice could be restored by the anti-oxidant, N-acetyl-l-cysteine, supporting that decreased Aco2 activity in HD is probably caused by increased oxidative damage. Decreased Aco2 activity was further found in the peripheral blood mononuclear cells (PBMC) of both HD patients and pre-symptomatic HD mutation (PreHD) carriers, while the decreased Aco2 protein level of PBMC was only present in HD patients. Aco2 activity correlated significantly with motor score, independence scale, and functional capacity of the Unified Huntington's Disease Rating Scale as well as disease duration. Our study provides a potential biomarker to assess the disease status of HD patients and PreHD carriers.

Keywords: Hdh(CAG)150 and R6/2 mice; Huntington’s disease; N-acetyl-l-cysteine; aconitase 2; biomarker; peripheral blood mononuclear cells.

MeSH terms

  • Acetylcysteine / pharmacology
  • Aconitate Hydratase / metabolism*
  • Age Factors
  • Animals
  • Blood Cells / enzymology*
  • Corpus Striatum / drug effects
  • Corpus Striatum / enzymology*
  • Corpus Striatum / physiopathology
  • Disease Models, Animal
  • Enzyme Activation
  • Genotype
  • Huntingtin Protein / genetics
  • Huntington Disease / enzymology*
  • Huntington Disease / physiopathology
  • Mice
  • Motor Activity / drug effects
  • Mutation
  • Trinucleotide Repeat Expansion


  • Huntingtin Protein
  • Aconitate Hydratase
  • aconitase 2, mouse
  • Acetylcysteine