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. 2018 Apr;144:71-75.
doi: 10.1016/j.pep.2017.11.003. Epub 2017 Nov 21.

High Level Expression and Characterization of Tannase tan7 Using Aspergillus Niger SH-2 With Low-Background Endogenous Secretory Proteins as the Host

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High Level Expression and Characterization of Tannase tan7 Using Aspergillus Niger SH-2 With Low-Background Endogenous Secretory Proteins as the Host

Fengling Liu et al. Protein Expr Purif. .

Abstract

Tannin acyl hydrolase (tannase, EC3.1.1.20) catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid. In this study, we reported that the overexpression, purification and characterization of an Aspergillus niger tannase. The tannase gene was cloned from A. niger SH-2 and expressed in the A. niger strain Bdel4 which is low-background of secreted proteins. The recombinant tannase was purified by desalting, followed by gel filtration for characterization. The tannase activity achieved 111.5 U/mL at 168 h, and the purity of the enzyme in the broth supernatant was estimated to be over 70%. The optimum temperature and pH of the recombinant tannase was ∼40 °C and 7.0, respectively. The tannase activity was inhibited by Mg2+, Ca2+, Cu2+, Ba2+, Ni2+ and EDTA, and was enhanced by Mn2+ and Co2+. Since A. niger is a GRAS microorganism, the recombinant tannase could be purification-free due to its high purity. The results of this study suggested that this recombinant strain could be subjected to large-scale production of A. niger tannase.

Keywords: Aspergillus niger; Expression; Purification; Tannin acyl hydrolase (Tannase).

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