Silencing of OsGRXS17 in rice improves drought stress tolerance by modulating ROS accumulation and stomatal closure

Sci Rep. 2017 Nov 21;7(1):15950. doi: 10.1038/s41598-017-16230-7.


Glutaredoxins (GRXs) modulate redox-dependent signaling pathways and have emerged as key mediators in plant responses to environmental stimuli. Here we report that RNAi-mediated suppression of Oryza sativa GRXS17 (OsGRXS17) improved drought tolerance in rice. Gene expression studies showed that OsGRXS17 was present throughout the plant and that transcript abundance increased in response to drought stress and abscisic acid (ABA) treatment. Localization studies, utilizing GFP-OsGRXS17 fusion proteins, indicated that OsGRXS17 resides in both the cytoplasm and the nuclear envelope. Under drought stress conditions, rice plants with reduced OsGRXS17 expression showed lower rates of water loss and stomatal conductance, higher relative water content, and enhanced survival compared to wild-type controls. Further characterization of the OsGRXS17 down-regulated plants revealed an elevation in H2O2 production within the guard cells, increased sensitivity to ABA, and a reduction in stomatal apertures. The findings demonstrate a critical link between OsGRXS17, the modulation of guard cell H2O2 concentrations, and stomatal closure, expanding our understanding of the mechanisms governing plant responses to drought.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Abscisic Acid / pharmacology
  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / genetics*
  • Amino Acid Sequence
  • Droughts*
  • Gene Expression Regulation, Plant / drug effects
  • Gene Silencing*
  • Genes, Plant
  • Hydrogen Peroxide / metabolism
  • Oryza / drug effects
  • Oryza / genetics
  • Oryza / physiology*
  • Phenotype
  • Plant Growth Regulators / pharmacology
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Stomata / cytology
  • Plant Stomata / drug effects
  • Plant Stomata / physiology*
  • Protein Domains
  • Reactive Oxygen Species / metabolism*
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics*


  • Plant Growth Regulators
  • Plant Proteins
  • Reactive Oxygen Species
  • Abscisic Acid
  • Hydrogen Peroxide