Temporally coordinated spiking activity of human induced pluripotent stem cell-derived neurons co-cultured with astrocytes

Biochem Biophys Res Commun. 2018 Jan 1;495(1):1028-1033. doi: 10.1016/j.bbrc.2017.11.115. Epub 2017 Nov 21.


In culture conditions, human induced-pluripotent stem cells (hiPSC)-derived neurons form synaptic connections with other cells and establish neuronal networks, which are expected to be an in vitro model system for drug discovery screening and toxicity testing. While early studies demonstrated effects of co-culture of hiPSC-derived neurons with astroglial cells on survival and maturation of hiPSC-derived neurons, the population spiking patterns of such hiPSC-derived neurons have not been fully characterized. In this study, we analyzed temporal spiking patterns of hiPSC-derived neurons recorded by a multi-electrode array system. We discovered that specific sets of hiPSC-derived neurons co-cultured with astrocytes showed more frequent and highly coherent non-random synchronized spike trains and more dynamic changes in overall spike patterns over time. These temporally coordinated spiking patterns are physiological signs of organized circuits of hiPSC-derived neurons and suggest benefits of co-culture of hiPSC-derived neurons with astrocytes.

Keywords: Astrocyte co-culture; Human induced-pluripotent stem cell-derived neurons; Multi-electrode array; Synchronization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Astrocytes / cytology
  • Astrocytes / physiology*
  • Biological Clocks / physiology*
  • Cell Communication / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Coculture Techniques / methods*
  • Computer Simulation
  • Humans
  • Models, Neurological*
  • Neurons / cytology
  • Neurons / physiology*
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / physiology
  • Time Factors