Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast

Michael Reidy

doi:10.1007/978-1-4939-7477-1_14

Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast

Methods Mol Biol. 2018:1709:189-198. doi: 10.1007/978-1-4939-7477-1_14.

Author

Michael Reidy¹

Affiliation

¹ Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA. michael.reidy@nih.gov.

PMID: 29177660
DOI: 10.1007/978-1-4939-7477-1_14

Abstract

Heat shock protein 90 (Hsp90) is an essential regulator of cellular function through activation of so-called client proteins. Hsp90 is currently a target for potential anti-cancer therapeutics. Assaying for defects in Hsp90 using budding yeast (Saccharomyces cerevisiae) is possible and efficient due to the high conservation between the human and yeast Hsp90 systems. Here, we present a method for the determination of Hsp90 activity indirectly through the activation of exogenously expressed glucocorticoid receptor, a natural client protein of Hsp90.

Keywords: Glucocorticoid receptor; Hsp90; Saccharomyces cerevisiae.

MeSH terms

HSP90 Heat-Shock Proteins / metabolism*
Molecular Biology / methods*
Receptors, Glucocorticoid / metabolism*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Signal Transduction*

Substances

HSP82 protein, S cerevisiae
HSP90 Heat-Shock Proteins
Receptors, Glucocorticoid
Saccharomyces cerevisiae Proteins