Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats

Int J Ophthalmol. 2017 Nov 18;10(11):1662-1668. doi: 10.18240/ijo.2017.11.05. eCollection 2017.


Aim: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats.

Methods: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ).The retina tissues were used to determine 8-hydroxy-2'-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px).

Results: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P=0.001, P<0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P=0.004, P<0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P≤0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P=0.010, P=0.042, respectively) but SOD activity was not statistically different (P=0.938).

Conclusion: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.

Keywords: antioxidant enzymes; diabetic retinopathy; immunohistochemical staining; oxidative stress; pomegranate juice.