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. 2017 Dec;54(13):4473-4483.
doi: 10.1007/s13197-017-2928-6. Epub 2017 Oct 20.

Characterization of probiotic potential of Bacillus species isolated from a traditional brine pickle

K Ragul  1 , Ibadondor Syiem  1 , K Sundar  1 , Prathapkumar H Shetty  1
Affiliations

Characterization of probiotic potential of Bacillus species isolated from a traditional brine pickle

K Ragul et al. J Food Sci Technol. 2017 Dec.

Abstract

Fermented foods are known for their potential as main source of probiotics. The present study aimed at investigating the probiotic properties of bacteria isolated from fermented mango pickle. Non-hemolytic fermenting microbiota isolated from mango pickle was screened in vitro for their basic probiotic properties such as acid tolerance, bile salt, lysozyme and salt tolerance. They were also tested for their beneficial characters like cholesterol removal, bacterial adhesion to hydrocarbons, auto-aggregation, antimicrobial activity, β-galactosidase activity, exopolysaccharide production and adhesion to HT-29 cell line. Outputs of these parameters were subjected to principal component analysis (PCA) and these results were interpreted to select prospective bacterial isolates that can be used as potential probiotics. Out of eight isolates, PUFSTP35 (Bacillus licheniformis), PUFSTP38 (Bacillus amyloliquefaciens) and PUFSTP39 (Bacillus subtilis) showed similar trend to Weissella cibaria (MTCC 9814) that was used as a reference strain for profiling probiotic properties. B. licheniformis PUFSTP35 from fermented mango pickle appear to be the most potential candidate for use as a beneficial probiotic.

Keywords: Bacillus sp.; Fermented food; Health benefits; Mango pickle; Probiotic properties.

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Conflict of interest statement

Authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
a Cholesterol removal by test isolates in de-Man Rogosa Sharpe broth at 37 °C and cholesterol removal was expressed in terms of percentage ± SE. b Adhesion of test isolates to human colon cell line HT-29 in de-Man Rogosa Sharpe agar at 37 °C and adhesion was expressed in terms of percentage ± SE. c Adhesion of selected bacterial strains on HT-29 cell line stained with Giemsa stain (40 ×)
Fig. 2
Fig. 2
a Graphical representation of the variables formed by PC1 and PC2 analyzed by principal component analysis. b Prediction of the test isolates and reference strain Weisella cibaria (SS1) in the plane of PC1 and PC2
Fig. 3
Fig. 3
UPGMA phylogenetic tree based on 16S rRNA sequences of selected isolates (*) with reference strains from NCBI database
See this image and copyright information in PMC

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