Studies of a glomerular permeability factor in patients with minimal-change nephrotic syndrome

Nephron. 1989;51(3):370-6. doi: 10.1159/000185325.


Peripheral blood mononuclear cells (PBMC) from patients with minimal-change nephrotic syndrome (MCNS) were tested for their ability to produce a factor which increases the urinary protein excretion levels of rats. It was shown that enhanced proteinuria can be produced in 8-hour urine specimens from rats by the injection of concentrated supernatants of cultured concanavalin-A-stimulated PBMC of patients with MCNS, but not from other nephrotics or normal subjects. The increase in urinary protein excretion was associated with a significant alteration of glomerular epithelial cells similar to that seen in MCNS. These results suggest that in MCNS, PBMC release a factor, which we termed a glomerular permeability factor (GPF), causing changes in glomerular permeability with resulting proteinuria.

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Concanavalin A / pharmacology
  • Female
  • Humans
  • Kidney Glomerulus / metabolism*
  • Kidney Glomerulus / ultrastructure
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Nephrosis, Lipoid / blood*
  • Permeability
  • Proteinuria / etiology
  • Proteinuria / pathology
  • Rats
  • Rats, Inbred Strains
  • Urine


  • Concanavalin A