Solvent saturation transfer to proteins (SSTP) for structural and functional characterization of proteins

J Biomol NMR. 2018 Jan;70(1):11-20. doi: 10.1007/s10858-017-0151-4. Epub 2017 Nov 30.

Abstract

Protein structure determination using NMR is dependent on experimentally acquired distance restraints. Often, however, an insufficient number of these restraints are available for determining a protein's correct fold, much less its detailed three-dimensional structure. In consideration of this problem, we propose a simple means to acquire supplemental structural restraints from protein surface accessibilities using solvent saturation transfer to proteins (SSTP), based on the principles of paramagnetic chemical-exchange saturation transfer. Here, we demonstrate the utility of SSTP in structure calculations of two proteins, TSG101 and ubiquitin. The observed SSTP was found to be directly proportional to solvent accessibility. Since SSTP does not involve the direct excitation of water, which compromises the analysis of protein protons entangled in the breadth of the water resonance, it has an advantage over conventional water-based magnetization transfers. Inclusion of structural restraints derived from SSTP improved both the precision and accuracy of the final protein structures in comparison to those determined by traditional approaches, when using minimal amounts of additional structural data. Furthermore, we show that SSTP can detect weak protein-protein interactions which are unobservable by chemical shift perturbations.

Keywords: CSP; NMR; ParaCEST; Structure refinements; TSG101; Ubiquitin; Yb-DOTAM.

MeSH terms

  • DNA-Binding Proteins / chemistry
  • Endosomal Sorting Complexes Required for Transport / chemistry
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation
  • Proteins / chemistry*
  • Solvents
  • Transcription Factors / chemistry
  • Ubiquitin / chemistry

Substances

  • DNA-Binding Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Proteins
  • Solvents
  • Transcription Factors
  • Tsg101 protein
  • Ubiquitin