Sulfanilic acid-modified chitosan mini-spheres and their application for lysozyme purification from egg white

Biotechnol Prog. 2018 Mar;34(2):387-396. doi: 10.1002/btpr.2588. Epub 2017 Dec 7.


A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g-1 while the dissociation constant was 0.074 ± 0.012 mg mL-1 . The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387-396, 2018.

Keywords: chitosan; chromatographic matrix; egg white lysozyme; multimodal ion exchange chromatography; sulfanilic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Buffers
  • Chitosan / chemistry*
  • Chromatography, High Pressure Liquid
  • Egg White / chemistry*
  • Magnetic Resonance Spectroscopy
  • Muramidase / isolation & purification*
  • Muramidase / metabolism
  • Osmolar Concentration
  • Sulfanilic Acids / chemistry*


  • Buffers
  • Sulfanilic Acids
  • Chitosan
  • Muramidase