EG-VEGF Maintenance Over Early Gestation to Develop a Pregnancy-Induced Hypertensive Animal Model

Methods Mol Biol. 2018;1710:317-324. doi: 10.1007/978-1-4939-7498-6_25.


During the last decade, multiple animal models have been developed to mimic hallmarks of pregnancy-induced hypertension (PIH) diseases, which include gestational hypertension, preeclampsia (PE), or eclampsia. Converging in vitro, ex vivo, and clinical studies from our group strongly suggested the potential involvement of the new angiogenic factor EG-VEGF (endocrine gland-derived-VEGF) in the development of PIH. Here, we described the protocol that served to demonstrate that maintenance of EG-VEGF production over 11.5 days post coitus (dpc) in the gravid mice caused the development of PIH. The developed model exhibited most hallmarks of preeclampsia.

Keywords: Animal model; EG-VEGF; Glomerulosclerosis; Hypertension; Invasion; Placenta; Preeclampsia; Pregnancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Female
  • Humans
  • Hypertension, Pregnancy-Induced / metabolism*
  • Hypertension, Pregnancy-Induced / pathology
  • Male
  • Mice
  • Pre-Eclampsia / metabolism
  • Pre-Eclampsia / pathology
  • Pregnancy
  • Trophoblasts / metabolism
  • Trophoblasts / pathology
  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / analysis
  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / metabolism*


  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived