Culture-independent genome sequencing of Coxiella burnetii from a native heart valve of a Tunisian patient with severe infective endocarditis

J Delaloye; T Pillonel; M Smaoui; A Znazen; L Abid; G Greub

doi:10.1016/j.nmni.2017.09.004

Culture-independent genome sequencing of Coxiella burnetii from a native heart valve of a Tunisian patient with severe infective endocarditis

New Microbes New Infect. 2017 Oct 10:21:31-35. doi: 10.1016/j.nmni.2017.09.004. eCollection 2018 Jan.

Authors

J Delaloye^{1

2}, T Pillonel¹, M Smaoui³, A Znazen³, L Abid⁴, G Greub¹

Affiliations

¹ Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
² Intensive Care Unit, Department of Intensive Care Medicine, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
³ Laboratory of Microbiology, CHU Habib Bourguiba Sfax, University of Sfax, Tunisia.
⁴ Department of Cardiology, Hedi Chaker University Hospital, University of Sfax, Sfax, Tunisia.

Abstract

We report draft genome of a Coxiella burnetii strain sequenced from the native valve of a patient presenting with severe endocarditis in Tunisia. The genome could be sequenced without a cellular or axenic culture step. The MST5 strain was demonstrated to be closely related to the published reference genome of C. burnetii CbuK_Q154.

Keywords: Cardiac valve; endocarditis; genomics; intracellular bacteria.