Extraction Method and Analysis of Cannabinoids in Cannabis Olive Oil Preparations

Planta Med. 2018 Mar;84(4):242-249. doi: 10.1055/s-0043-123074. Epub 2017 Dec 4.

Abstract

Recently, an increasing number of pharmacists had to supply medicinal products based on Cannabis sativa L. (Cannabaceae), prescribed by physicians to individual patients. Cannabis olive oil preparation is the first choice as a concentrated extract of cannabinoids, even though standardized operative conditions for obtaining it are still not available. In this work, the impact of temperature and extraction time on the concentration of active principles was studied to harmonize the different compounding methods, optimize the extraction process, and reduce the variability among preparations. Moreover, starting from the cannabis inflorescence, the effect of temperature on tetrahydrocannabinolic acid decarboxylation was evaluated. For the analysis, a GC/MS method, as suggested by the Italian Ministry of Health, and a GC/flame ionization detection method were developed, validated, and compared.

MeSH terms

  • Cannabinoids / analysis*
  • Cannabis / anatomy & histology
  • Cannabis / chemistry*
  • Cannabis / ultrastructure
  • Flame Ionization / methods
  • Gas Chromatography-Mass Spectrometry / methods
  • Hot Temperature
  • Microscopy
  • Microscopy, Electron, Scanning
  • Olive Oil / chemistry*
  • Olive Oil / therapeutic use
  • Plant Extracts / chemistry*
  • Plant Leaves / anatomy & histology
  • Plant Leaves / chemistry
  • Plant Leaves / ultrastructure

Substances

  • Cannabinoids
  • Olive Oil
  • Plant Extracts