Anti-Candida Activity of Bursera Morelensis Ramirez Essential Oil and Two Compounds, α-Pinene and γ-Terpinene-An In Vitro Study

Molecules. 2017 Dec 5;22(12):2095. doi: 10.3390/molecules22122095.

Abstract

The candidiasis caused by C. albicans is a public health problem. The abuse of antifungals has contributed to the development of resistance. B. morelensis has demonstrated antibacterial and antifungal activities. In this work the activity of the essential oil of B. morelensis was evaluated and for its two pure compounds with analysis of the different mechanisms of pathogenesis important for C. albicans. The essential oil was obtained by the hydro-distillation method and analyzed using GC-MS. The anti-Candida activity was compared between to essential oil, α-Pinene and γ-Terpinene. GC-MS of the essential oil demonstrated the presence of 13 compounds. The essential oil showed antifungal activity against four C. albicans strains. The most sensitive strain was C. albicans 14065 (MFC 2.0 mg/mL and MIC50 0.125 mg/mL) with α-Pinene and γ-Terpinene having MFCs of 4.0 and 16.0 mg/mL respectively. The essential oil inhibited the growth of the germ tube in 87.94% (8.0 mg/mL). Furthermore, it was observed that the essential oil diminishes the transcription of the gene INT1. This work provides evidence that confirms the anti-Candida activity of the B. morelensis essential oil and its effect on the growth of the germ tube and transcription of the gene INT1.

Keywords: Bursera morelensis; Candida albicans; burseraceae; α-pinene; γ-terpinene.

MeSH terms

  • Antifungal Agents / chemistry
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / pharmacology*
  • Bicyclic Monoterpenes
  • Bursera / chemistry*
  • Candida / drug effects*
  • Candida / genetics
  • Candida / growth & development
  • Candida / metabolism
  • Cell Adhesion Molecules / antagonists & inhibitors
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism
  • Cyclohexane Monoterpenes
  • Fungal Proteins / antagonists & inhibitors
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gas Chromatography-Mass Spectrometry
  • Gene Expression
  • Microbial Sensitivity Tests
  • Monoterpenes / isolation & purification
  • Monoterpenes / pharmacology*
  • Oils, Volatile / chemistry
  • Oils, Volatile / isolation & purification
  • Oils, Volatile / pharmacology
  • Plant Extracts / chemistry
  • Plant Oils / chemistry
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology
  • Spores, Fungal / drug effects*
  • Spores, Fungal / genetics
  • Spores, Fungal / growth & development
  • Spores, Fungal / metabolism

Substances

  • Antifungal Agents
  • Bicyclic Monoterpenes
  • Cell Adhesion Molecules
  • Cyclohexane Monoterpenes
  • Fungal Proteins
  • Monoterpenes
  • Oils, Volatile
  • Plant Extracts
  • Plant Oils
  • alphaINT1 protein, Candida albicans
  • gamma-terpinene
  • alpha-pinene