MicroRNA-630 inhibits breast cancer progression by directly targeting BMI1

Exp Cell Res. 2018 Jan 15;362(2):378-385. doi: 10.1016/j.yexcr.2017.11.039. Epub 2017 Dec 5.

Abstract

MicroRNAs (miRNAs) play critical roles in breast cancer cell biological processes, including proliferation and apoptosis by inhibiting the expression of their target genes. Herein, we reported that miR-630 overexpression initiates apoptosis, blocks cell cycle progression and suppresses cell proliferation in breast cancer cells. Furthermore, BMI1, a member of polycomb group family, was identified as a direct target of miR-630, and there was a negative correlation between the expression levels of BMI1 and miR-630 in human breast cancer samples. With a series of biology approaches, subsequently, we proved that BMI1 was a functional downstream target of miR-630 and mediated the property of miR-630-dependent inhibition of breast cancer progression. Taken together, these findings provide further evidence on the tumor-suppression function of miR-630 in breast cancer, and clarify BMI1 as a novel functional target gene of miR-630.

Keywords: Apoptosis; BMI1; Breast cancer; miR-630.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • MicroRNAs / genetics*
  • Mitogen-Activated Protein Kinase 7 / genetics*

Substances

  • MIRN630 microRNA, human
  • MicroRNAs
  • MAPK7 protein, human
  • Mitogen-Activated Protein Kinase 7