Mitochondrial tRNA Ala C5601T mutation may modulate the clinical expression of tRNA Met A4435G mutation in a Han Chinese family with hypertension

Clin Exp Hypertens. 2018;40(6):595-600. doi: 10.1080/10641963.2017.1411497. Epub 2017 Dec 6.

Abstract

Mutations in mitochondrial DNA, especially in mitochondrial tRNA (mt-tRNA) genes, are the important causes for maternally inherited hypertension. In this study, we reported the clinical, genetic, and molecular characterization of a Han Chinese family with hypertension. Most strikingly, this family exhibited a high penetrance and expressivity of hypertension. Sequence analysis of the complete mt-tRNA genes showed the presence of tRNAMet A4435G and tRNAAla C5601T mutations. The A4435G had already been reported as a pathogenic mutation associated with hypertension; in addition, the C5601T mutation, which was located at the highly conserved nucleotide of T arm of tRNAAla, created a novel Watson-Crick base pairing and may result in failure of tRNA metabolism. Moreover, bioinformatics analysis indicated that the C5601T mutation altered the secondary structure of tRNAAla. Thus, the mitochondrial dysfunction, caused by the A4435G mutation, may be worsened by the C5601T mutation. Taken together, our data indicated that the co-occurrence of the A4435G and C5601T mutations may account for the high penetrance and expressivity of hypertension in this family. Therefore, our study provided novel insight into the pathophysiology of maternally inherited hypertension.

Keywords: A4435G mutation; C5601T mutation; Hypertension; mt-tRNA.

MeSH terms

  • Adult
  • Aged
  • Asians / genetics*
  • DNA, Mitochondrial / genetics*
  • Family
  • Female
  • Gene Expression Regulation / genetics*
  • Humans
  • Hypertension / genetics*
  • Male
  • Middle Aged
  • Mutation / genetics*
  • Pedigree
  • RNA, Transfer, Ala / genetics*
  • RNA, Transfer, Met / genetics*

Substances

  • DNA, Mitochondrial
  • RNA, Transfer, Ala
  • RNA, Transfer, Met