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. 2017 Dec 7;18(12):2652.
doi: 10.3390/ijms18122652.

Joint Effects of PON1 Polymorphisms and Vegetable Intake on Ischemic Stroke: A Family-Based Case Control Study

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Free PMC article

Joint Effects of PON1 Polymorphisms and Vegetable Intake on Ischemic Stroke: A Family-Based Case Control Study

Juan Juan et al. Int J Mol Sci. .
Free PMC article

Abstract

Paraoxonase 1 gene (PON1) polymorphisms and dietary vegetable and fruit intake are both established determinants of ischemic stroke (IS). However, little is known about whether these factors jointly influence the risk of IS. We analyzed the main effects of PON1, as well as the interactions between PON1 and dietary vegetable or fruit intake with the risk of total IS and its subtypes in a family-based case-control study conducted among 2158 Chinese participants (1007 IS cases and 1151 IS-free controls) from 918 families. Conditional logistic regression models, with each family as a stratum, were used to examine the association between rs662 and IS. Gene-diet interactions were tested by including a cross-product term of dietary vegetable or fruit intake by rs662_G allele count in the models. Each copy of the PON1 rs662_G allele was associated with 28% higher risk of total IS (p = 0.008) and 32% higher risk of large artery atherosclerosis subtype (LAA) (p = 0.01). We observed an interaction between rs662 and vegetable intake for both total IS (p = 0.006) and LAA (p = 0.02) after adjustment for covariates. Individuals who carry the rs662_A allele may benefit to a greater extent from intake of vegetables and thus be more effectively protected from ischemic stroke, whereas carriers of the G allele may still remain at greater risk for ischemic stroke due to their genetic backgrounds even when they consume a high level of vegetables. More studies are needed to replicate our findings among other populations.

Keywords: PON1; family-based case-control study; gene-diet interaction; ischemic stroke; ischemic stroke subtypes; vegetable intake.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Interactions between PON1 rs662_G allele numbers and standardized vegetable or fruit intake for total ischemic stroke and LAA. Interactions between PON1 rs662_G allele count and vegetable intake for total ischemic stroke (A) and LAA (B) and interactions between PON1 rs662_G allele count and fruit intake for total ischemic stroke (C) and LAA (D). Blue line represents those who are homozygous for the A allele (A/A), red line represents heterozygous (A/G), and green line represents homozygous for the G allele (G/G). The Y-axis shows the natural log transformed ORs. The X-axis shows the mean-centered and standardized intake levels of vegetable or fruit (participants with the highest and lowest 1% of vegetable intake levels were excluded to minimize the potential impact of outliers). LAA, large artery atherosclerosis; OR, odds ratio.

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