Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb

Neuron. 2017 Dec 6;96(5):1139-1152.e7. doi: 10.1016/j.neuron.2017.11.008.


Mitral and tufted (M/T) cells in the olfactory bulb produce rich temporal patterns of activity in response to different odors. However, it remains unknown how these temporal patterns are generated and how they are utilized in olfaction. Here we show that temporal patterning effectively discriminates between the two sensory modalities detected by olfactory sensory neurons (OSNs): odor and airflow-driven mechanical signals. Sniff-induced mechanosensation generates glomerulus-specific oscillatory activity in M/T cells, whose phase was invariant across airflow speed. In contrast, odor stimulation caused phase shifts (phase coding). We also found that odor-evoked phase shifts are concentration invariant and stable across multiple sniff cycles, contrary to the labile nature of rate coding. The loss of oscillatory mechanosensation impaired the precision and stability of phase coding, demonstrating its role in olfaction. We propose that phase, not rate, coding is a robust encoding strategy of odor identity and is ensured by airflow-induced mechanosensation in OSNs.

Keywords: concentration invariance; mechanosensation; mitral/tufted cells; olfaction; olfactory bulb; olfactory sensory neurons; oscillations; phase coding; sniffing; temporal coding.

MeSH terms

  • Absorptiometry, Photon
  • Animals
  • Behavior, Animal / physiology
  • Immunohistochemistry
  • Mechanotransduction, Cellular / physiology*
  • Mice
  • Neuroimaging
  • Odorants
  • Olfactory Bulb / physiology*
  • Olfactory Pathways / physiology
  • Olfactory Receptor Neurons / physiology
  • Smell / physiology*
  • Tracheotomy