GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis

Methods Mol Biol. 2018:1713:255-267. doi: 10.1007/978-1-4939-7507-5_19.


Brain glucose sensing plays an essential role in the regulation of energy homeostasis. Recent publications report that neurons expressing glucose transporter GLUT2 act as glucose sensors in different regions of the brain and contribute to the control of glucose homeostasis and feeding behavior. In this chapter we describe the methods used to explore glucose sensing in genetically tagged GLUT2-expressing neurons with slice electrophysiology.

Keywords: Electrophysiology; GLUT2; Glucose sensing; Juxtacellular; Mice; NTS; Neurons; PVT; Whole-cell.

MeSH terms

  • Animals
  • Brain / physiology*
  • Electrophysiological Phenomena*
  • Gene Expression*
  • Glucose / metabolism*
  • Glucose Transporter Type 2 / genetics*
  • Glucose Transporter Type 2 / metabolism
  • Male
  • Mice
  • Molecular Imaging
  • Neurons / physiology*
  • Patch-Clamp Techniques


  • Glucose Transporter Type 2
  • Glucose