Single-shot speckle correlation fluorescence microscopy in thick scattering tissue with image reconstruction priors

Julie Chang; Gordon Wetzstein

doi:10.1002/jbio.201700224

Single-shot speckle correlation fluorescence microscopy in thick scattering tissue with image reconstruction priors

J Biophotonics. 2018 Mar;11(3). doi: 10.1002/jbio.201700224. Epub 2018 Jan 30.

Authors

Julie Chang¹, Gordon Wetzstein²

Affiliations

¹ Bioengineering Department, Stanford University, Stanford, CA, USA.
² Electrical Engineering Department, Stanford University, Stanford, CA, USA.

PMID: 29219256
DOI: 10.1002/jbio.201700224

Abstract

Deep tissue imaging in the multiple scattering regime remains at the frontier of fluorescence microscopy. Speckle correlation imaging (SCI) can computationally uncover objects hidden behind a scattering layer, but has only been demonstrated with scattered laser illumination and in geometries where the scatterer is in the far field of the target object. Here, SCI is extended to imaging a planar fluorescent signal at the back surface of a 500-μm-thick slice of mouse brain. The object is reconstructed from a single snapshot through phase retrieval using a proximal algorithm that easily incorporates image priors. Simulations and experiments demonstrate improved image recovery with this approach compared to the conventional SCI algorithm.

Keywords: computational imaging; deep tissue imaging; fluorescence microscopy; phase retrieval; speckle correlation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Animals
Artifacts
Image Processing, Computer-Assisted*
Mice
Microscopy, Fluorescence*
Optical Phenomena*