Hypoglycemic and hypolipidemic effects of anthocyanins extract from black soybean seed coat in high fat diet and streptozotocin-induced diabetic mice

Food Funct. 2018 Jan 24;9(1):426-439. doi: 10.1039/c7fo00983f.


Black soybean seed coat extract (BSSCE) is a rich source of anthocyanins with multiple health effects. This study was aimed at investigating the composition and hypoglycemic and hypolipidemic effects of BSSCE in vitro and in a high-fat diet and streptozotocin (STZ)-induced diabetic mice. The anthocyanins of BSSCE were identified as cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, and peonidin-3-O-glucoside by HPLC-MS. Results demonstrated that BSSCE exhibited strong inhibitory activities for α-amylase, potent inhibition activity against lipid accumulation in HepG2 cells and protection effect on H2O2-induced oxidative stress-damaged HepG2 cells. The food and water intake, body weight loss, blood glucose and insulin level of BSSCE treatment group were found to be significantly reduced when compared with those of diabetic mice group (p < 0.05). The fasting blood glucose level and insulin level of the BSSCE 400 mg kg-1 group mice significantly decreased by 47.97% and 46.49%, respectively. The oral glucose tolerance and activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) notably improved (p < 0.05). BSSCE could also ameliorate the atherogenic dyslipidaemia of diabetic mice by remarkably decreasing the content of total cholesterol (T-CHO), total triglyceride (TG), and non-esterified fatty acid (NEFA) and increasing the content of high-density lipoprotein cholesterol (HDL-c) (p < 0.05). BSSCE could protect against liver, kidney and pancreas damages in diabetic mice. This study suggested that cyanidin-3-O-glucoside contributed to BSSCE-induced hypoglycemia and hypolipidemia effects in type 2 diabetes mellitus (T2DM), and BSSCE might be a promising functional food or medicine for T2DM treatment.

MeSH terms

  • Animals
  • Anthocyanins / administration & dosage*
  • Anthocyanins / chemistry
  • Blood Glucose / metabolism
  • Catalase / metabolism
  • Diabetes Mellitus, Type 2 / chemically induced
  • Diabetes Mellitus, Type 2 / drug therapy*
  • Diabetes Mellitus, Type 2 / metabolism
  • Glutathione Peroxidase / metabolism
  • Humans
  • Hypoglycemic Agents / administration & dosage*
  • Hypoglycemic Agents / chemistry
  • Hypolipidemic Agents / administration & dosage*
  • Hypolipidemic Agents / chemistry
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Oxidative Stress / drug effects
  • Plant Extracts / administration & dosage*
  • Plant Extracts / chemistry
  • Seeds / chemistry
  • Soybeans / chemistry*
  • Streptozocin / adverse effects
  • Superoxide Dismutase / metabolism
  • Triglycerides / metabolism


  • Anthocyanins
  • Blood Glucose
  • Hypoglycemic Agents
  • Hypolipidemic Agents
  • Plant Extracts
  • Triglycerides
  • Streptozocin
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase