Biodegradation and detoxification of Direct Black G textile dye by a newly isolated thermophilic microflora

Yan Chen; Linlin Feng; Hanguang Li; Yuanxiu Wang; Guotao Chen; Qinghua Zhang

doi:10.1016/j.biortech.2017.11.092

Biodegradation and detoxification of Direct Black G textile dye by a newly isolated thermophilic microflora

Bioresour Technol. 2018 Feb:250:650-657. doi: 10.1016/j.biortech.2017.11.092. Epub 2017 Nov 29.

Authors

Yan Chen¹, Linlin Feng¹, Hanguang Li¹, Yuanxiu Wang¹, Guotao Chen¹, Qinghua Zhang²

Affiliations

¹ College of Bioscience and Biotechnology, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, PR China; Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, PR China.
² College of Bioscience and Biotechnology, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, PR China; Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, PR China. Electronic address: zqh_net@163.com.

PMID: 29220809
DOI: 10.1016/j.biortech.2017.11.092

Abstract

The biodegradation and detoxification of azo dye - Direct Black G (DBG) with a newly isolated thermophilic microflora was investigated in the present study. It was found this microflora can decolorize DBG at a wide range of pH from 5 to 10, and grow well under high concentration of dye (600 mg·L^-1) and salinity (50 g·L^-1). Its decolorization ratio could reach 97% with 8 h of incubation at optimal conditions. The induction of laccase, manganese peroxidase, lignin peroxidase and azoreductase suggests their synergetic involvements in the degradation process of DBG. In addition, the phytotoxicity analysis indicated the thermophilic microflora converted toxic dye DBG into low toxicity metabolites. PCR-DGGE analysis revealed that there are nine different bacteria presented in this microflora. Furthermore, a new degradation pathway of DBG degradation by this microflora was proposed based on the intermediates identified by LC-ESI-MS/MS.

Keywords: Azo dyes; Decolorization; Degradation pathway; PCR-DGGE; Thermophilic microflora.

MeSH terms

Azo Compounds*
Biodegradation, Environmental*
Color
Coloring Agents
Laccase
Tandem Mass Spectrometry
Textile Industry

Substances

Azo Compounds
Coloring Agents
Laccase