Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses

Methods Mol Biol. 2018;1727:201-215. doi: 10.1007/978-1-4939-7571-6_15.


Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.

Keywords: Calyx of held; Capacitance measurements; Giant synapse; Neuronal cell culture; Neurotrophin-3; Synapse imaging; Synaptic vesicles.

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cells, Cultured
  • Electrophysiological Phenomena
  • Mice
  • Molecular Imaging
  • Neurons / cytology*
  • Synapses / physiology
  • Synapses / ultrastructure*