Salusin-α Attenuates Inflammatory Responses in Vascular Endothelial Cells

Biochemistry (Mosc). 2017 Nov;82(11):1314-1323. doi: 10.1134/S0006297917110098.


Atherosclerosis accounts for numerous cardiovascular diseases, and cytokines have a critical role in acceleration or suppression of disease. Salusin-α presents a new class of bioactive peptides that can have anti-atherogenic properties. Therefore, the effects of salusin-α on the expression of some pro- and anti-inflammatory cytokines and on TNF-α-induced inflammatory responses in human umbilical vein endothelial cells (HUVECs) were examined. The involvement of the NF-κB pathway in effects of salusin-α in HUVECs was checked using Bay 11-7082 as an NF-κB inhibitor. The mRNA expression of pro-inflammatory cytokines including IL-6, IL-8, and IL-18 and anti-inflammatory cytokine IL-1Ra was assessed by real-time PCR. The protein levels of cytokines were measured by the ELISA method. Salusin-α suppressed both mRNA and protein expression of pro-inflammatory cytokines and induced mRNA and protein expression of IL-1Ra in HUVECs. Salusin-α suppressed TNF-α-induced inflammatory responses in HUVECs. The down-regulatory or up-regulatory effects of salusin-α on expression of cytokines could not be influenced by Bay 11-7082 pretreatment. Our findings indicate anti-inflammatory effects of salusin-α and suggest a novel peptide-based therapeutic strategy for atherosclerosis.

MeSH terms

  • Anti-Inflammatory Agents / pharmacology
  • Atherosclerosis / pathology
  • Cells, Cultured
  • Cytokines / analysis
  • Cytokines / genetics
  • Endothelial Cells / pathology*
  • Gene Expression Regulation / drug effects
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Inflammation / drug therapy*
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Interleukin 1 Receptor Antagonist Protein / analysis
  • Interleukin 1 Receptor Antagonist Protein / genetics
  • NF-kappa B / metabolism
  • RNA, Messenger / analysis
  • RNA, Messenger / drug effects
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / drug effects


  • Anti-Inflammatory Agents
  • Cytokines
  • Intercellular Signaling Peptides and Proteins
  • Interleukin 1 Receptor Antagonist Protein
  • NF-kappa B
  • RNA, Messenger
  • TOR2A protein, human
  • Tumor Necrosis Factor-alpha