A novel peptide-based vaccine candidate with protective efficacy against influenza A in a mouse model

Virology. 2018 Feb;515:21-28. doi: 10.1016/j.virol.2017.11.018. Epub 2017 Dec 7.

Abstract

Current influenza vaccines mainly induce antibody responses to the variable hemagglutinin proteins of the virus strains included in the vaccine. Instead, a broadly protective influenza vaccine should aim at inducing antibody- and/or cell-mediated immunity against conserved viral proteins. Vacc-FLU is a peptide based vaccine combining conserved B and T cell epitopes. Peptide selection was done using a proprietary peptide design platform technology focusing on responses to human leukocyte antigen (HLA)-restricted epitopes. Immunization of wild-type mice and mice transgenic for HLA-A2.1 with the peptide mix successfully induced both humoral and cell mediated immune responses. Partial protection from severe weight loss upon challenge was observed in both mouse strains but was stronger and observed at lower vaccine doses in transgenic mice. Our results show that the Vacc-FLU peptide mix is capable of inducing IFNγ-producing T cells and antibody-producing B cells which can protect from severe disease symptoms upon infection.

Keywords: Cell mediated immunity; Humoral immunity; Influenza; Peptides; Universal vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Epitopes, T-Lymphocyte / immunology
  • Female
  • HLA-A2 Antigen
  • Humans
  • Immunity, Cellular*
  • Immunity, Humoral*
  • Immunization
  • Influenza A virus / immunology*
  • Influenza Vaccines / immunology*
  • Influenza, Human / prevention & control*
  • Influenza, Human / virology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Peptides / immunology*
  • Random Allocation

Substances

  • Epitopes, T-Lymphocyte
  • HLA-A2 Antigen
  • Influenza Vaccines
  • Peptides