Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

Methods Mol Biol. 2018;1712:217-238. doi: 10.1007/978-1-4939-7514-3_14.

Abstract

The paucity of pathogenic T cells in circulating blood limits the information delivered by bulk analysis. Toward diagnosis and monitoring of treatments of autoimmune diseases, we have devised single-cell analysis approaches capable of identifying and characterizing rare circulating CD4 T cells.

Keywords: Autoimmunity; CD4 T cells; Microfluidics; Peripheral blood; Single-cell analysis; TCR sequences.

MeSH terms

  • Animals
  • Autoimmune Diseases / diagnosis*
  • Autoimmunity / genetics
  • CD4-Positive T-Lymphocytes / immunology*
  • Gene Expression Profiling*
  • Gene Library
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • Mice
  • Real-Time Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Single-Cell Analysis
  • Statistics as Topic

Substances

  • Receptors, Antigen, T-Cell, alpha-beta