Raspberry ketone protects against isoproterenol-induced myocardial infarction in rats

Life Sci. 2018 Feb 1;194:205-212. doi: 10.1016/j.lfs.2017.12.013. Epub 2017 Dec 7.

Abstract

Aim: The cardioprotective role of raspberry ketone (RK) against isoproterenol (ISO)-induced myocardial infarction (MI) in rats was assessed.

Materials and methods: Rats were randomly divided into Group I - Vehicle control; Group II - Toxic control ISO (85mg/kg, s.c.); Group III, IV and V - RK (50, 100 and 200mg/kg, respectively) with ISO; Group VI- RK (200mg/kg) alone; Group VII - Propranolol (10mg/kg) with ISO; and Group VIII - Propranolol (10mg/kg) alone. After twenty-four hours of the last dose, animals were sacrificed and creatine kinase-MB, lactate dehydrogenase, total cholesterol, triglycerides, high-density-lipoprotein, low-density-lipoprotein, very-low-density-lipoprotein, malondialdehyde, reduced glutathione, superoxide dismutase, catalase, Na+, K+-ATPase, nitric oxide, histopathological and immunohistochemical analysis (tumor necrosis factor-α and inducible nitric oxide synthase) were performed.

Key findings: Treatment with ISO significantly deviated the biochemical parameters from the normal levels, which were considerably restored by RK at 100 and 200mg/kg doses. 50mg/kg dose, however, did not demonstrate any significant cardioprotective action. The histopathological and immunohistochemical analysis further substantiated these findings.

Significance: Our study showed a dose-dependent reduction in oxidative stress, inflammation and dyslipidemia by RK in ISO-intoxicated rats, which signifies that RK from the European red raspberry plant might be a valuable entity for the management of MI.

Keywords: Inflammation; Isoproterenol; Myocardial infarction; Nitric oxide; Oxidative stress; Raspberry ketone; Rubus idaeus.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Antioxidants / therapeutic use*
  • Butanones / chemistry
  • Butanones / therapeutic use*
  • Cardiotonic Agents / therapeutic use*
  • Isoproterenol
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / pathology
  • Myocardial Infarction / prevention & control*
  • Myocardium / pathology
  • Oxidative Stress / drug effects
  • PPAR alpha / agonists
  • Rats
  • Rats, Wistar
  • Rubus / chemistry

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Butanones
  • Cardiotonic Agents
  • PPAR alpha
  • raspberry ketone
  • Isoproterenol