Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2017 Dec 8;71(0):1005-1014.
doi: 10.5604/01.3001.0010.6696.

Structure of Bacterial Chromosome: An Analysis of DNA-protein Interactions in Vivo

Affiliations
Free article
Review

Structure of Bacterial Chromosome: An Analysis of DNA-protein Interactions in Vivo

Joanna Hołówka et al. Postepy Hig Med Dosw (Online). .
Free article

Abstract

According to recent reports, bacterial chromosomes exhibit a hierarchical organization. The number of proteins that bind DNA are responsible for local and global organization of the DNA ensuring proper chromosome compaction. Advanced molecular biology techniques combined with high-throughput DNA sequencing methods allow a precise analysis of bacterial chromosome structures on a local and global scale. Methods such as in vivo footprinting and ChIP-seq allow to map binding sites of analyzed proteins in certain chromosomal regions or along the whole chromosome while analysis of the spatial interactions on global scale could be performed by 3C techniques. Additional insight into complex structures created by chromosome-organizing proteins is provided by high-resolution fluorescence microscopy techniques.

Keywords: 3C; 4C; 5C; ChIP-seq; Hi-C; bacterial chromosome; fluorescence microscopy; in vivo footprinting; nucleoid associated proteins (NAP).

Similar articles

See all similar articles

MeSH terms

LinkOut - more resources

Feedback