A Proposal for Automated Background Removal of Bio-Raman Data

Anal Sci. 2017;33(12):1323-1325. doi: 10.2116/analsci.33.1323.


The subtraction of background components from observed spectra is essential in performing multivariable analysis, frequently applied to Raman spectra of cells. The subtraction procedure, however, is manual and time consuming, especially for big data. Here, we propose an automated method for removing background information from measured spectra of cells, exhibiting the theoretical framework and an experimental application.

Keywords: Raman spectra; background removal; cell; quartz substrate; subtraction.

MeSH terms

  • Algorithms
  • Automation
  • HeLa Cells
  • Humans
  • Spectrum Analysis, Raman*
  • Statistics as Topic / methods*