A CBL-interacting protein kinase TaCIPK27 confers drought tolerance and exogenous ABA sensitivity in transgenic Arabidopsis

Yan Wang; Tingting Li; Shanita Judith John; Mingjie Chen; Junli Chang; Guangxiao Yang; Guangyuan He

doi:10.1016/j.plaphy.2017.11.019

A CBL-interacting protein kinase TaCIPK27 confers drought tolerance and exogenous ABA sensitivity in transgenic Arabidopsis

Plant Physiol Biochem. 2018 Feb:123:103-113. doi: 10.1016/j.plaphy.2017.11.019. Epub 2017 Dec 1.

Authors

Yan Wang¹, Tingting Li², Shanita Judith John³, Mingjie Chen⁴, Junli Chang⁵, Guangxiao Yang⁶, Guangyuan He⁷

Affiliations

¹ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: 875643001@qq.com.
² The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: 1532975716@qq.com.
³ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: 1615240070@qq.com.
⁴ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: cmj@hust.edu.cn.
⁵ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: cjl@hust.edu.cn.
⁶ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: ygx@hust.edu.cn.
⁷ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: hegy@hust.edu.cn.

PMID: 29227949
DOI: 10.1016/j.plaphy.2017.11.019

Abstract

Drought is one of the major environmental stresses to plants. The calcium sensor, calcineurin B-like (CBL) proteins, and their interacting protein kinases (CIPK) play important roles in responding to abiotic stresses. In this study, we functionally characterized a CIPK gene from Triticum aestivum designated TaCIPK27. The transcriptional levels of TaCIPK27 were increased both in roots and leaves after treatment with polyethylene glycol 8000, abscisic acid and H₂O₂. Besides, TaCIPK27 interacted with AtCBL1, AtCBL3, AtCBL4, AtCBL5 and AtCBL9 in yeast two-hybrid assays. Ectopic overexpression of TaCIPK27 positively regulates drought tolerance in transgenic Arabidopsis compared with controls, which was demonstrated by seed germination and survival rates experiments, as well as the detection of physiological indices including ion leakage, malonic dialdehyde and H₂O₂ contents and antioxidant enzyme activities under normal and drought conditions. Moreover, higher concentration of endogenous abscisic acid was detected under drought in TaCIPK27 transgenic plants. In addition, TaCIPK27 transgenic plants were more sensitive to exogenous abscisic acid treatment at seed germination and seedling stage. The expression levels of somedrought stress and abscisic acid related genes were up-regulated in TaCIPK27 transgenic plants. The results suggest that TaCIPK27 functions as a positive regulator under drought partly in an ABA-dependent pathway.

Keywords: Abscisic acid; Arabidopsis; Drought tolerance; Stomatal movement; TaCIPK27; Wheat.

MeSH terms

Abscisic Acid / pharmacology*
Arabidopsis* / enzymology
Arabidopsis* / genetics
Dehydration / enzymology
Dehydration / genetics
Disease Resistance* / drug effects
Disease Resistance* / genetics
Plant Proteins* / genetics
Plant Proteins* / metabolism
Plants, Genetically Modified* / enzymology
Plants, Genetically Modified* / genetics
Protein Serine-Threonine Kinases* / genetics
Protein Serine-Threonine Kinases* / metabolism
Triticum / enzymology
Triticum / genetics*

Substances

Plant Proteins
Abscisic Acid
Protein Serine-Threonine Kinases