Cardiovascular disease risk factors and oxidative stress among premenopausal women

Free Radic Biol Med. 2018 Feb 1;115:246-251. doi: 10.1016/j.freeradbiomed.2017.12.006. Epub 2017 Dec 9.


Oxidative stress is one hypothesized mechanism linking anthropometric, behavioral, and medical risk factors with cardiovascular disease (CVD). We evaluated cross-sectional associations between CVD risk factors and biomarkers of oxidative stress, and investigated these biomarkers as predictors of incident diabetes and hypertension among premenopausal women. F2-isoprostane (F2-IsoP) and metabolite (15-F2t-IsoP-M), reliable biomarkers of oxidative stress, were measured in urine samples collected at enrollment from 897 premenopausal women (ages 35-54) enrolled in the Sister Study cohort without a CVD history. Blood pressure, waist circumference, and body mass index (BMI) were measured at enrollment by trained study personnel. Diabetes and cigarette smoking were self-reported via enrollment questionnaires. Over a maximum follow-up of 11.5 years, participants self-reported incident diabetes and hypertension diagnoses on mailed questionnaires. In cross-sectional analyses, both F2-IsoP and 15-F2t-IsoP-M were positively associated with BMI, waist circumference, diastolic blood pressure, and current smoking. F2-IsoP was elevated among those with diabetes, and 15-F2t-IsoP-M increased with higher systolic blood pressure. Prospective analyses suggested an increased hypertension risk among those with elevated 15-F2t-IsoP-M (highest vs. lowest quartile: hazard ratio=2.34; 95% CI: 1.20-4.56). Our results suggest that urinary F2-IsoP and 15-F2t-IsoP-M are positively associated with adiposity measures, blood pressure, and cigarette smoking. Further investigation is warranted to evaluate 15-F2t-IsoP-M as a predictor of hypertension.

Keywords: Body mass index; Cardiovascular disease; F(2)-isoprostanes; Hypertension; Oxidative stress.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biomarkers / metabolism*
  • Biometry
  • Blood Pressure
  • Cardiovascular Diseases / diagnosis
  • Cardiovascular Diseases / epidemiology*
  • Cardiovascular Diseases / metabolism
  • Cohort Studies
  • Cross-Sectional Studies
  • Dinoprost / analogs & derivatives*
  • Dinoprost / metabolism
  • F2-Isoprostanes / metabolism*
  • Female
  • Follow-Up Studies
  • Humans
  • Middle Aged
  • Oxidative Stress
  • Predictive Value of Tests
  • Premenopause*
  • Prognosis
  • Risk Factors
  • United States / epidemiology


  • Biomarkers
  • F2-Isoprostanes
  • 8-epi-prostaglandin F2alpha
  • Dinoprost