ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing

Cytometry B Clin Cytom. 2018 Jan;94(1):23-48. doi: 10.1002/cyto.b.21610.


Since publication in 2010 of the International Clinical Cytometry Society (ICCS) Consensus Guidelines for detection of Paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometery, a great deal of work has been performed to develop, optimize, and validate a number of high-sensitivity assays to detect PNH phenotypes in both red blood cells (RBCs) and white blood cells (WBCs, neutrophils, and monocytes). This section (Part 2) of the updated ICCS PNH Consensus Guidelines will focus on specific instrument setup for these PNH assays, the identification and proper testing of appropriate antibody conjugates and combinations therof, and basic assay design. © 2017 International Clinical Cytometry Society.

Keywords: PNH; high sensitivity flow cytometry; paroxysmal nocturnal hemoglobinuria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Consensus
  • Erythrocytes / metabolism
  • Flow Cytometry / standards*
  • Hemoglobinuria, Paroxysmal / diagnosis*
  • Hemoglobinuria, Paroxysmal / metabolism*
  • Humans
  • Leukocyte Count / methods
  • Leukocytes / metabolism
  • Monocytes / metabolism
  • Neutrophils / metabolism