Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)

PLoS One. 2017 Dec 13;12(12):e0188779. doi: 10.1371/journal.pone.0188779. eCollection 2017.

Abstract

Introduction: Wounds are a common health problem. Coffee is widely consumed and its oil contains essential fatty acids. We evaluated the local (skin) and systemic effects associated with the topical use of coffee oils in rats.

Methods: Punch skin wounds (6 mm) incisions were generated on the backs of 75 rats. Saline (SS), mineral oil (MO), green coffee oil (GCO), roasted coffee oil (RCO), green coffee ground oil (GCGO) or roasted coffee ground oil (RCGO) were topically applied to the wounds. Healing was evaluated by visual and histological/morphometric optical microscopy examination; second harmonics generation (SHG) microscopy, wound tissue q-PCR (values in fold-change) and blood serum (ELISA, values in pg/mL).

Results: RCO treated animals presented faster wound healing (0.986 vs. 0.422), higher mRNA expression of IGF-1 (2.78 vs. 1.00, p = 0.01), IL-6 (10.72 vs. 1.00, p = 0.001) and IL-23 (4.10 vs. 1.2, p = 0.05) in early stages of wound healing; higher IL-12 (3.32 vs. 1.00, p = 0.05) in the later stages; and lower serum levels of IFN-γ (11.97 vs. 196.45, p = 0.01). GCO treatment led to higher mRNA expression of IL-6 (day 2: 7.94 vs. 1.00, p = 0.001 and day 4: 6.90 vs. 1.00, p = 0.01) and IL-23 (7.93 vs. 1.20, p = 0.001) in the early stages. The RCO treatment also produced higher serum IFN-α levels throughout the experiment (day 2: 52.53 vs. 21.20; day 4: 46.98 vs.21.56; day 10: 83.61 vs. 25.69, p = 0.05) and lower levels of IL-4 (day 4: 0.9 vs.13.36, p = 0.01), adiponectin (day 10: 8,367.47 vs. 16,526.38, p = 0.001) and IFN-γ (day 4: 43.03 vs.196.45, p = 0.05). The SHG analysis showed a higher collagen density in the RCO and GCO treatments (p = 0.05).

Conclusion: Topical treatment with coffee oils led to systemic actions and faster wound healing in rats. Further studies should be performed are necessary to assess the safety of topical vegetal oil use for skin lesions.

MeSH terms

  • Animals
  • Coffee*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Models, Biological*
  • Plant Oils / administration & dosage*
  • Plant Oils / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Real-Time Polymerase Chain Reaction
  • Skin / injuries*
  • Wound Healing / drug effects*

Substances

  • Coffee
  • Plant Oils

Grant support

This work was supported by grant # 07/59319-5 Sao Paulo Research Foundation - Fapesp - http://www.fapesp.br/en/ - NBM; grant # 015/2014 - Fundo de Apoio à Dermatologia do Estado de São Paulo "Sebastião Sampaio" - Funadersp - http://www.sbd-sp.org.br/funadersp.php - PENFV; grant # 043/2016 - Fundo de Apoio à Dermatologia do Estado de São Paulo "Sebastião Sampaio" - Funadersp - http://www.sbd-sp.org.br/funadersp.php - PENFV; grant # 01P-04520-2013 - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Capes - http://www.capes.gov.br/ - BGL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.