Konjac Ceramide (kCer) Regulates NGF-Induced Neurite Outgrowth via the Sema3A Signaling Pathway

J Oleo Sci. 2018 Jan 1;67(1):77-86. doi: 10.5650/jos.ess17141. Epub 2017 Dec 14.

Abstract

The tuber of the konjac plant is a source enriched with GlcCer (kGlcCer), and has been used as a dietary supplement to improve the dry skin and itching that are caused by a deficiency of epidermal ceramide. Previously, we showed chemoenzymatically prepared konjac ceramide has a neurite-outgrowth inhibitory effect that is very similar to that of Sema3A and is not seen with animal-type ceramides. While, it has been unclear whether kCer may act on Sema3A or TrkA signaling pathway. In the present study, we showed kCer induces phosphorylation of CRMP2 and microtubules depolymerization via Sema3A signaling pathway not TrkA. It is concluded that kCer may be a potential Sema3A-like agonist that activates Sema3A signaling pathway directly.

Keywords: CRMP2; NGF; ceramide; konjac; neurite outgrowth; semaphorin 3A.

MeSH terms

  • Amorphophallus
  • Animals
  • Antibodies / pharmacology
  • Carbazoles / pharmacology
  • Cell Line, Tumor
  • Glucosylceramides / pharmacology*
  • Indole Alkaloids / pharmacology
  • Mice
  • Microtubules / drug effects
  • Microtubules / ultrastructure
  • Nerve Growth Factor / pharmacology
  • Nerve Tissue Proteins / metabolism
  • Neuronal Outgrowth / drug effects*
  • Neuropilin-1 / immunology
  • Phosphorylation / drug effects
  • Rats
  • Semaphorin-3A / agonists*
  • Semaphorin-3A / metabolism
  • Signal Transduction / drug effects*

Substances

  • Antibodies
  • Carbazoles
  • Glucosylceramides
  • Indole Alkaloids
  • Nerve Tissue Proteins
  • Sema3a protein, rat
  • Semaphorin-3A
  • collapsin response mediator protein-2
  • Neuropilin-1
  • Nerve Growth Factor
  • staurosporine aglycone