Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage

Laryngoscope. 2018 Apr;128(4):E123-E129. doi: 10.1002/lary.26980. Epub 2017 Dec 14.


Objectives/hypothesis: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model.

Study design: Basic science experimental design.

Methods: ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features.

Results: In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results.

Conclusions: This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement.

Level of evidence: NA. Laryngoscope, 128:E123-E129, 2018.

Keywords: Larynx; adipose stem cell; cartilage; chondrocytes; collagen; oligomer; tissue engineering.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipose Tissue / cytology
  • Adipose Tissue / transplantation*
  • Animals
  • Laryngeal Cartilages / transplantation*
  • Laryngectomy / methods*
  • Male
  • Mesenchymal Stem Cell Transplantation / methods*
  • Mesenchymal Stem Cells / cytology
  • Rats
  • Rats, Inbred F344
  • Tissue Engineering / methods*
  • Tissue Scaffolds