Diazepam binding inhibitor-like immunoreactivity(51-70): distribution in human brain, spinal cord and peripheral tissues

Brain Res. 1989 Feb 13;479(2):300-5. doi: 10.1016/0006-8993(89)91632-6.


We have used a specific radioimmunoassay to describe the distribution of diazepam binding inhibitor-like immunoreactivity (DBI-IR(51-70) in human post-mortem tissues. In brain, highest concentrations were found in the cerebellum, amygdala, hippocampus, hypothalamus and substantia nigra. In the spinal cord, DBI-IR(51-70) was evenly distributed. In peripheral tissues, highest concentrations were found in the liver and kidney. Chromatographic analysis revealed several molecular forms of DBI-IR(51-70) the major form being of greater molecular weight and hydrophobicity than the synthetic fragment peptide. In peripheral tissues, but not in the CNS, a small peak of immunoreactivity was indistinguishable from the synthetic peptide. DBI-IR(51-70) is therefore widespread, but tissue processing may be different.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Brain / metabolism*
  • Diazepam Binding Inhibitor
  • Female
  • Humans
  • Kidney / metabolism*
  • Liver / metabolism*
  • Male
  • Middle Aged
  • Neuropeptides / metabolism*
  • Peptide Fragments / pharmacology*
  • Spinal Cord / metabolism*


  • Diazepam Binding Inhibitor
  • Neuropeptides
  • Peptide Fragments