Synthesis of the major inducible heat shock protein in rat hippocampus after neonatal hypoxia-ischemia

Exp Neurol. 1989 Apr;104(1):28-31. doi: 10.1016/0014-4886(89)90005-8.

Abstract

Rats aged 7 days were exposed to 3.5 h of cerebral hypoxia-ischemia produced by unilateral common carotid artery ligation combined with hypoxia (8% oxygen). The major inducible heat-shock protein, HSP-68, was synthesized in ipsilateral but not contralateral (control) hippocampus during early recovery (1 and 3 h). HSP-68 synthesis was not detected during longer recovery periods. The presence of HSP-68 was confirmed by Western blotting and immunostaining with a polyclonal antibody to HSP-68.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Autoradiography
  • Carotid Arteries / physiology
  • Functional Laterality
  • Heat-Shock Proteins / biosynthesis*
  • Heat-Shock Proteins / isolation & purification
  • Hippocampus / metabolism*
  • Hypoxia / metabolism*
  • Ischemic Attack, Transient / metabolism*
  • Methionine / metabolism
  • Molecular Weight
  • Rats
  • Rats, Inbred Strains
  • Sulfur Radioisotopes

Substances

  • Heat-Shock Proteins
  • Sulfur Radioisotopes
  • Methionine