Labeling and identifying cell-specific proteomes in the mouse brain

Nat Biotechnol. 2018 Feb;36(2):156-159. doi: 10.1038/nbt.4056. Epub 2017 Dec 18.


We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids
  • Amino Acyl-tRNA Synthetases / genetics*
  • Animals
  • Brain / metabolism*
  • Gene Expression Regulation, Enzymologic / genetics
  • Mice
  • Neuroglia / metabolism
  • Neurons / metabolism*
  • Proteome / genetics*
  • RNA, Transfer / genetics


  • Amino Acids
  • Proteome
  • RNA, Transfer
  • Amino Acyl-tRNA Synthetases